#MAKE UNIT DISK GRAPH GIVEN SET OF POINTS HOW TO#
The following procedure describes how to use it: This command uses the Particle Analyzer↑ to extract sets of coordinate data from digitized line graphs. Select Gels ▷↑Label Peaks to label each measured peak with its size as a percent of the total size of the measured peaks.If necessary, scroll the image vertically by holding down the space bar and dragging. For each peak, measure the size by clicking inside the peak with the Wand Tool↑.To access to all the lanes, it may be necessary to scroll the image vertically using the Scrolling Tool↑ (Hold down the space bar to temporarily switch to this tool). Note that you can hold Shift to constrain lines to be either horizontal or vertical. Use the Straight Line Selection Tool↑ to draw base lines and/or drop lines so that each peak of interest defines a closed area (ImageJ will automatically switch to the Straight Line tool).Select Gels ▷↑Plot Lanes ( 3 ) to generate the lane profile plots.Repeat the previous step for each remaining lane.The selected lane is outlined and labelled, and ‘Lane n selected’ is displayed in the status bar. Move the rectangular selection right to the next lane (or down if the lanes are horizontal) and select Gels ▷↑Select Next Lane ( 2 ).Select Gels ▷↑Select First Lane ( 1 ) and the lane will be outlined and ‘Lane 1 selected’ displayed in the status bar.
This should be the left most lane if the lanes are vertical or the top lane if the lanes are horizontal.
Use the rectangular selection tool to outline the first lane.Note that a copy of the gel image with the lane outlines can be created at any point using the Image ▷Overlay ▷ Flatten ↑ command. For practice, refer to the video tutorial on the ImageJ wikipage and use the File ▷ Open Samples ▷↑Gel sample image (1-D gel) to perform the following steps.
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